Biochemistry (Set-3)

Some important Biochemistry questions for the 1st year MBBS , BAMS (Ayurveda) and Nursing students that might be asked in the Terminal or University / Proffesional Exams.

Section A    (50 Marks)

Q1.Long Answer Question:     (1+4+10 = 15 marks)

A 70 year old male in good health previously, presented with sudden pain in the right great toe in the night after a bout of alcohol consumption. On examination, he had mild fever, his right great toe was wollen warm, red and tender Serum uric acid was 10

(a) What is the probable diagnosis?.
(6) Discus the biochemical basis of this condition along with different types of it.
(c) Enumerate the pathway of purine nucleotide catabolism

Q2. Short Answer Question:         (7×5 = 35 marks)

1) Define Induction & repression. Describe briefly about Lac Operon.
2) Explain briefly about Post Transcriptional Modification.
3) Discuss about Recombinant DNA technology & its applications.
4) Define Mutation. Describe about types of Mutation with examples.
5) Explain briefly about PCR technique & its applications.
6) Describe Structure of Immunoglobulins.
7) Name drugs which inhibits translation & describe their mechanism of action

Section B       (50 Marks)

Q1. A 52 year old man with presents to the emergency department history in semiconscious state, he had diabetes mellitus since 20 years. He has an SpO2 of 98% on room air and is obviously tachypnes on exam with what appears to be Kunamaal’s respirations. A room air arterial blood gas is performed and reveal:      (3×5 = 15 Marks)

pH: 7.15 (735-745);  PCO: 21(35-45 mm of Hg);  Potassiam: 4.0 (3.5-5.5mEq/L) ; Sodium: 140.0 (135-1555mEq/L); HCO: 16.022-26 mm of Hg);  Chloride: 104 (95-1055 mEq/L); Blood glucose: 504 mg/dl 

(a) Which type acid-base imbalance is evident from it this ABG report?
(b) Define anion gap.
(c) Calculate the anion gap in this patient.
(d) Discuss all the possible differential diagnosis of underlying disorder in this patient? 
(e) Describe the mechanism of compensation in this disorder?

2. Write short notes       (4×5 = 20 Marks)

(a) Free radical and antioxid.
(b) Phase II xenobiotic.
(c) Liver function test.
(d) Tumor suppressor gene.
(e) Cardiac markers

3. Short answer questions justify :-     (5×3 = 15 Marks)

(a) Blood creatinine more better investigation than urine creatinine.
(b) Vitamin C act as Pro-oxidant in higher doses. 
(c) Serum is better than plasma for biochemical investigation.
(d) Lambert-beer law used for biochemical parameter.
(e) Enumerate body fluid in our body.

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